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つくばリポジトリ (Tulips-R) >
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Cryobiology >
Please use this identifier to cite or link to this item:
http://hdl.handle.net/2241/98218
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| Title: | Freeze-dried platelets promote hepatocyte proliferation in mice |
| Authors: | Hoshi, Reiko
Murata, Soichiro
Matsuo, Ryota
Myronovych, Andriy
Hashimoto, Ikuka
Ikeda, Hiroshi
Ohkohchi, Nobuhiro
大河内, 信弘
村田, 聡一郎 |
| Issue Date: | Dec-2007 |
| Publisher: | Elsevier |
| Journal Title: | Cryobiology |
| Volume: | 55 |
| Issue: | 3 |
| Start Page: | 255 |
| End Page: | 260 |
| DOI: | 10.1016/j.cryobiol.2007.08.007 |
| PMID: | 17936259 |
| Abstract: | In recent years, platelets are reported to promote liver, as well as bone regeneration and dermal wound healing. Platelets are required not only for thrombocytopenia treating but also for regenerative therapy. Platelets cannot be stored beyond three days, therefore, shortage of fresh platelets sometimes occurs. To preserve platelets for a long duration without degrading growth factors, a freeze-dried technique is required. We report here that platelets can be preserved by freeze-drying, using a programmed freezing method to avoid intracellular ice crystal formation. Freeze-dried platelets kept their morphological countenance and response with the agonist of thrombin was well maintained. Freeze-dried platelets stored adenine nucleotides, PDGF, and IGF-1 the same as those of fresh platelets. Freeze dried platelets also preserved their proliferative effect on hepatocytes identical to that of fresh platelets. These results of our study suggest that freeze dried platelets will obviate the storage problem of fresh platelets. |
| URI: | http://hdl.handle.net/2241/98218 |
| Rights: | © 2007 Elsevier Inc. |
| Text Version: | author |
| Appears in Collections: | Cryobiology
大河内 信弘 (Ohkohchi Nobuhiro)
村田 聡一郎 (Murata Soichiro)
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