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|Title: ||Proteomic profiles of embryonic development in the ascidian Ciona intestinalis|
|Authors: ||Nomura, Mamoru|
|Issue Date: ||Jan-2009 |
|Publisher: ||Elsevier Inc.|
|Journal Title: ||Developmental biology|
|Start Page: ||468|
|End Page: ||481|
|Abstract: ||We report here proteomics-based protein profiles of three embryonic stages of the ascidian Ciona intestinalis.
Two-dimensional gel electrophoresis revealed 416, 539, and 695 protein spots in the unfertilized eggs, 16
cell-stage embryos, and tadpole larvae, respectively. Comparative and quantitative analyses of the spot
patterns identified proteins showing an increase or decrease in amount during embryonic development.
Protein identification by MALDI-TOF/MS indicated not only the abundance and importance of metabolic
enzymes and translation elongation factors but also the functional importance of actin-binding proteins and
molecular chaperones during ascidian development. Global changes in spots for vitellogenin-like protein
suggested post-translational modification or proteolytic digestion of this protein during embryogenesis.
Comparison between mRNA and protein levels among unfertilized eggs, 16 cell-stage embryos and tadpole
larvae indicated nonparallel expression patterns of genes and proteins. Ascidians provide an excellent system
for studying gene expression and cell differentiation during development, and the present study should shed
light on the associated molecular mechanism at the protein level.|
|Rights: ||© 2008 Elsevier Inc|
|Text Version: ||author|
|Appears in Collections:||Developmental biology|
稲葉 一男 (Inaba Kazuo)
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